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KMID : 0359319960360020359
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Korean Journal of Veterinary Research
1996 Volume.36 No. 2 p.359 ~ p.369
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Effect on lymphocyte subpopulations of Quil A - ISCOMs with recombinant Aujeszkay´s disease virus ( ADV ) gp50 , gⅢ and α - ADV protein
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이성일/Lee, Sung Il
박용호/문진산/안수환/구복경/정석찬/현방훈/Park, Yong Ho/Moon, Jin San/An, Soo Hwan/Ku, Bok Gyeong/Jung, Suk Chan/Hyun, Bang Hun
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Abstract
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An effective candidate subunit vaccine was prepared by using the immunostimulating complexs(ISCOMs) with Quil A and recombinant protein(gp50, gIII and inactive α-ADV) Aujeszky´s disease vims(ADV). The weaned pigs were twice immunized with a ADV-ISCOMs, and followed by intramuscular challenge with 1×10⁴ TCID-50 ADV(strain Yangsan). The unvaccinated pigs were also challenged with same dose of ADV. At 5 days after challenge, the control pigs have developed ADV clinical signs. Whereas, the vaccinated pigs protected them from ADV-induced acute symptoms and death. Also, to identify the lymphocyte subpopulation in peripheral blood with pigs from ADV-ISCOMs vaccinated and control group, lymphocyte reacted with a panel of monoclonal antibodies which are specific to swine leukocyte surface antigens and assayed by the flow cytometry. MHC class I, CD2, CD8, N cells, CD11a, and CD45 antigen positive cells were decreased after inoculating virulent ADV Yangsan strain in control group. The data indicated that ISCOMs technique was useful in ADV subunit vaccine preparation and demonstrated the importance of gp50, gIII as a component of ADV vaccine.
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KEYWORD
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